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Cryo-electron Microscopy Support Specialist

Norwich, Norfolk NR4 7UH   •  £33,050 to £40,750 per annum depending on qualifications and experience  •  Permanent, Full time

Applications are invited for a Cryo-electron Microscopy Support Specialist to join the Bioimaging team at the John Innes Centre.

About the John Innes Centre:

The John Innes Centre is an independent, international centre of excellence in plant and microbial sciences. We nurture a creative, curiosity-led approach to answering fundamental questions in bioscience, and translate that knowledge into societal benefits. Our strategic vision, Healthy Plants, Healthy People, Healthy Planet, sets out our ambitious long-term goals for the game changing impact of our science globally.

Our employees enjoy access to state-of-the-art technology and a diverse range of specialist training opportunities, including support for leadership and management. Click here to find out more about working at the John Innes Centre.

About the Bioimaging Team:

Bioimaging is a state-of-the-art microscopy facility supporting world-class science at the John Innes Centre. The platform offers a wide range of imaging applications in both light and electron microscopy. The team can offer a comprehensive service, including imaging and custom sample preparation.

The role:

The John Innes Centre (JIC) is looking for an enthusiastic individual to provide support in cryo-EM, working with biological samples, in collaboration with multiple research groups within the JIC and The Sainsbury Laboratory, and sometimes from the wider Norwich Research Park (NRP). The post holder will be using a FEI Talos F200C (200kV, field emission, transmission electron microscope) with a newly installed Falcon 4i direct electron detector, Ceta-D camera for micro-ED and a Gatan OneView camera. For sample prep, we have a Vitrobot cryo-plunge device.

The post holder will be a permanent member of the Bioimaging team, using their knowledge and expertise in cryo-TEM to complement the Facility’s TEM, SEM and optical imaging skill-set.

Duties will involve using routine methods and developing new ones and applying them to a diverse range of research projects, day-to-day maintenance and operation of the instruments, sample preparation and data analysis to assist with manuscript submission. Techniques to be used include negative staining, cryo-EM single particle analysis, cryo-electron tomography and micro-ED. Activities may also include occasional support for other electron microscopy applications such as cryo-SEM.

The ideal candidate:

Candidates must be educated to at least BSc level or equivalent, and preferably a Phd, to be considered for the role. The applicant will ideally have extensive practical experience of using transmission electron microscopy. Experience in cryo-TEM is essential for this role.

Experience in operating FEI equipment is highly desirable, but training on our equipment can be provided. It would be beneficial if the post holder has experience of data processing for single particle analysis, such as the use of RELION, or the ability and willingness to learn these skills. A broad knowledge of other biological sample preparation techniques for EM is useful and there will be an opportunity for expanding their skill set in these areas.

Excellent communication and interpersonal skills are essential, as is a keen interest in biological science. An enthusiastic and motivated attitude is important, along with good time management and organisational skills. The successful candidate will have proven ability to work closely with researchers to understand their needs and translate these into EM approaches and applications.

Additional information:

Salary on appointment will be within the range £33,050 to £40,750 per annum depending on qualifications and experience. This is a full-time post offered on an indefinite basis.

We may be able to contribute towards relocation costs if required.

For further information and details of how to apply, please visit our website http://jobs.jic.ac.uk or contact the Human Resources team on 01603 450814 or nbi.recruitment@nbi.ac.uk quoting reference 1004412.

We are an equal opportunities employer, actively supporting inclusivity and diversity. As a Disability Confident organisation, we guarantee to offer an interview to all disabled applicants who meet the essential criteria for this vacancy. We are proud to hold a prestigious Gold Athena SWAN award in recognition of our inclusive culture, commitment and good practices towards advancing of gender equality. We offer an exciting, stimulating, diverse research environment and actively promote a family friendly workplace. The Institute is also a member of Stonewall’s Diversity Champions programme.

The John Innes Centre is a registered charity (No. 223852) grant-aided by the Biotechnology and Biological Sciences Research Council.

The closing date for applications will be 7 April 2023


Related skills: Biology, Life Science, Microbiology, Laboratory, Research

  • Employer: John Innes Centre
  • Contact: Human Resources
  • Phone: 01603 450814
  • Reference: 1004412
  • Posted: 9th March 2023
  • Expires: 7th April 2023

John Innes Centre

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