• We use cookies to help make our website work. LEARN MORE

Senior Scientist - CRISPR

Oxfordshire   •  £40000 - £45000 per annum  •  Permanent, Full time


An innovative start-up has an exciting opportunity for a Senior CRISPR Scientist to join its team. You will be responsible for CRISPR development and as a senior scientist, will be the go-to expert for gene editing., both single locus and genome-wide. To be considered you must have a PhD in a relevant subject and have in-depth experience of complex CRISPR assay design & delivery including but not limited to CRISPRko, CRISPRa/i or base editing.

Responsibilities

As a senior scientist responsible for CRISPR development you will be the go-to expert for gene editing., both single locus and genome-wide. Your experience in cell biology, molecular biology and cellular engineering will support our research programme and the delivery of company-wide milestones. Reporting to the Director of Cell Biology you will work with a team of scientists and the wider R&D department.

  • Design, develop and implement gene editing technologies, processes and applications
  • Responsible for the delivery of pooled CRISPR screening and base editing
  • Ability to work closely with Bioinformatician in the design and analysis of CRISPR experiments
  • Generation of Cas9 expressing cell lines, production of pooled lentivirus libraries and optimisation of screening conditions
  • Execution of pooled CRISPR screens and preparation of samples for NGS
  • Hit validation studies and stem cell differentiation
  • Create Standard operation procedures and document experiments using electronic laboratory notebook system
  • Engage with other teams and with external collaborators
  • Prepare and deliver technical report to the R&D team and senior management
  • Keep up to date with field developments and apply these to improve experimental design and assay performance
  • General ad-hoc duties as required to support the departmental and organisation requirements

Qualifications & Experience:

  • A PhD in a relevant area of Biological Sciences or related field
  • ≥ 5 years of post-doctoral or industry experience in cell line engineering and CRISPR editing

Experience:

  • In-depth experience of complex CRISPR assay design & delivery including but not limited to CRISPRko, CRISPRa/i or base editing
  • Experience in using key CRISPR bioinformatic tools and analysing CRISPR data (single gene level and multiplexed editing, eg from CRISPR screens)
  • Proven experience and track record in executing techniques in molecular assays and molecular biology (qRT-PCR, scRNA seq, ddPCR, cloning, NGS)
  • Experience in the maintenance and differentiation of stem cells
  • Experience and knowledge of cellular assays and cell phenotyping (FACS, IHC, clonogenic, cytotoxicity and viability).
  • Familiarity with the implementation, development and use of automation to support medium- throughput gene editing
  • Highly organised, self-motivated, and capable of working both independently and collaboratively; in a fast-paced team environment with shifting priorities
  • A proven history of independent investigations and innovative thinking


You must be eligible to work in the UK. Learn more

  • Agency: Cranleigh Scientific
  • Reference: 6892
  • Posted: 30th April 2021
  • Expires: 28th May 2021

Cranleigh Scientific

Share this job: